Hotspot

rbcL

Genome · 64415-65881 · 1467 bp

Back to Gracilaria

Pi 0.0649

MarkerSeek score 80.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: Genome
length_bp: 1467
variable_sites: 328
pi: 0.0649
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CCTCTGGTTTAGCTGTTGCA	ACACCAAGGAGCCCAAAAAT	50.0	45.0	58.1	57.9	2351-2406 bp	0.647059	72.5
rbcL_p2	CCTCTGGTTTAGCTGTTGCA	TGCAACCATACGACATGGAC	50.0	50.0	58.1	58.3	2372-2427 bp	0.647059	71.2
rbcL_p3	GACCCACCAGTTCTTGTTGT	ACACCAAGGAGCCCAAAAAT	50.0	45.0	57.9	57.9	2552-2564 bp	0.235294	55.4
rbcL_p4	GACCCACCAGTTCTTGTTGT	GTGCAACCATACGACATGGA	50.0	50.0	57.9	58.3	2574-2586 bp	0.235294	54.0
rbcL_p5	GACCCACCAGTTCTTGTTGT	TGCAACCATACGACATGGAC	50.0	50.0	57.9	58.3	2573-2585 bp	0.235294	54.0