Hotspot

orf671

Genome · 72044-74059 · 2016 bp

Back to Gracilaria

Pi 0.192
MarkerSeek score 83.40
Primer available yes
Label orf671

Feature Metadata

label_name
orf671
feature_type
gene
region
Genome
length_bp
2016
variable_sites
1288
pi
0.192
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf671_p1 ATCAGAAAAGCCTTGGCTGT AATTATCCTCTGCTGGTGGT 45.0 45.0 57.7 56.2 2934 bp 0.058824 25.5
orf671_p2 ATCAGAAAAGCCTTGGCTGT AAATTATCCTCTGCTGGTGGT 45.0 42.9 57.7 57.0 2935 bp 0.058824 23.7
orf671_p3 ATCAGAAAAGCCTTGGCTGT ACCAAATTATCCTCTGCTGGT 45.0 42.9 57.7 57.0 2938 bp 0.058824 23.7
orf671_p4 TGATCAGAAAAGCCTTGGCT AATTATCCTCTGCTGGTGGT 45.0 45.0 57.4 56.2 2936 bp 0.058824 23.2
orf671_p5 TGATCAGAAAAGCCTTGGCT AAATTATCCTCTGCTGGTGGT 45.0 42.9 57.4 57.0 2937 bp 0.058824 21.4