Hotspot

orf196

Genome · 69783-70373 · 591 bp

Back to Gracilaria

Pi 0.144

MarkerSeek score 84.20

Primer available yes

Label orf196

Feature Metadata

label_name: orf196
feature_type: gene
region: Genome
length_bp: 591
variable_sites: 284
pi: 0.144
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf196_p1	GCTAGAGGCTGTACATCGTT	TCCTTCAGCTGTTGCAACTT	50.0	45.0	57.1	57.9	1376 bp	0.058824	34.9
orf196_p2	GCTAGAGGCTGTACATCGTT	GACTCATGTGTGACCCGTTT	50.0	50.0	57.1	58.1	1261 bp	0.058824	34.4
orf196_p3	GCTAGAGGCTGTACATCGTT	ACTCATGTGTGACCCGTTTT	50.0	45.0	57.1	57.7	1260 bp	0.058824	32.6
orf196_p4	GCTAGAGGCTGTACATCGTT	TTCTCCTTCAGCTGTTGCAA	50.0	45.0	57.1	57.7	1379 bp	0.058824	32.6
orf196_p5	GCTAGAGGCTGTACATCGTT	TTTCTCCTTCAGCTGTTGCA	50.0	45.0	57.1	57.7	1380 bp	0.058824	32.6