Hotspot

orf190

Genome · 68995-69567 · 573 bp

Back to Gracilaria

Pi 0.181

MarkerSeek score 84.10

Primer available yes

Label orf190

Feature Metadata

label_name: orf190
feature_type: gene
region: Genome
length_bp: 573
variable_sites: 327
pi: 0.181
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf190_p1	GAACCAGCGACGTCCTAAAT	AACGATGTACAGCCTCTAGC	50.0	50.0	58.0	57.1	859 bp	0.058824	35.3
orf190_p2	GAGAAATGCATCTGGCTGGA	AACGATGTACAGCCTCTAGC	50.0	50.0	58.0	57.1	882 bp	0.058824	35.0
orf190_p3	AGGTGAGAAATGCATCTGGC	AACGATGTACAGCCTCTAGC	50.0	50.0	58.2	57.1	886 bp	0.058824	33.4
orf190_p4	GGTGAGAAATGCATCTGGCT	AACGATGTACAGCCTCTAGC	50.0	50.0	58.2	57.1	885 bp	0.058824	33.4
orf190_p5	TGAGAAATGCATCTGGCTGG	AACGATGTACAGCCTCTAGC	50.0	50.0	58.2	57.1	883 bp	0.058824	33.4