Hotspot

psbD-ompR

Genome · 176715-178345 · 1631 bp

Back to Gracilaria

Pi 0.357

MarkerSeek score 76.00

Primer available yes

Label psbD-ompR

Feature Metadata

label_name: psbD-ompR
feature_type: igs
region: Genome
length_bp: 1631
variable_sites: 1229
pi: 0.357
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-ompR_p1	GCTCCTCCAAGTATTCCAGC	GCAGGAGATGGAGAAGAAGC	55.0	55.0	58.0	58.0	2336-2998 bp	0.529412	72.5
psbD-ompR_p2	GCTCCTCCAAGTATTCCAGC	GATGTTGTTAGCGCAGGAGA	55.0	50.0	58.0	58.0	2348-2942 bp	0.470588	70.4
psbD-ompR_p3	AAGAAGTTGCAGCCTTCCAA	GATGTTGTTAGCGCAGGAGA	45.0	50.0	57.9	58.0	2488 bp	0.058824	41.7
psbD-ompR_p4	AGAAGTTGCAGCCTTCCAAA	GATGTTGTTAGCGCAGGAGA	45.0	50.0	57.9	58.0	2487 bp	0.058824	41.7
psbD-ompR_p5	AGAAGTTGCAGCCTTCCAAA	GCAGGAGATGGAGAAGAAGC	45.0	55.0	57.9	58.0	2475 bp	0.058824	41.4