Hotspot

trnR(ccg)-orf441

Genome · 58320-59091 · 772 bp

Back to Gracilaria

Pi 0.156
MarkerSeek score 83.40
Primer available yes
Label trnR(ccg)-orf441

Feature Metadata

label_name
trnR(ccg)-orf441
feature_type
igs
region
Genome
length_bp
772
variable_sites
371
pi
0.156
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(ccg)-orf441_p1 CCGATTCTGAAAGTCGAGGG AGCCCTACTGAAATCAAGCA 55.0 45.0 58.1 56.8 1000-1104 bp 0.470588 60.8
trnR(ccg)-orf441_p2 GTCGAGGGTTCAAGTCCTTC AGCCCTACTGAAATCAAGCA 55.0 45.0 57.9 56.8 988-1092 bp 0.470588 60.7
trnR(ccg)-orf441_p3 CCGATTCTGAAAGTCGAGGG ACCAGACAGTAAAGCCCTACT 55.0 47.6 58.1 58.1 1091-1095 bp 0.117647 39.5
trnR(ccg)-orf441_p4 GTCGAGGGTTCAAGTCCTTC ACCAGACAGTAAAGCCCTACT 55.0 47.6 57.9 58.1 1079-1083 bp 0.117647 39.5
trnR(ccg)-orf441_p5 CCGATTCTGAAAGTCGAGGG TCTACCAGACAGTAAAGCCCT 55.0 47.6 58.1 57.8 1094-1098 bp 0.117647 38.8