Hotspot

ycf15-trnL-CAA

IRa · 137727-138475 · 749 bp

Back to Goudaea

Pi 0

MarkerSeek score 61.60

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRa
length_bp: 749
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	GGCGAACAACCAGAGTACTC	AAATGGTAGACACGCGAGAC	55.0	50.0	58.3	58.0	913-935 bp	1.0	79.5
ycf15-trnL-CAA_p2	GGCGAACAACCAGAGTACTC	TGAAATGGTAGACACGCGAG	55.0	50.0	58.3	58.0	915-937 bp	1.0	79.5
ycf15-trnL-CAA_p3	GCGAACAACCAGAGTACTCA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.6	58.0	912-934 bp	1.0	78.4
ycf15-trnL-CAA_p4	GCGAACAACCAGAGTACTCA	TGAAATGGTAGACACGCGAG	50.0	50.0	57.6	58.0	914-936 bp	1.0	78.4
ycf15-trnL-CAA_p5	GGCGAACAACCAGAGTACTC	TGGTGAAATGGTAGACACGC	55.0	50.0	58.3	58.2	918-940 bp	1.0	78.0