Hotspot

psbZ-trnG-GCC

LSC · 78773-79089 · 317 bp

Back to Goudaea

Pi 0.00315

MarkerSeek score 50.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 317
variable_sites: 1
pi: 0.00315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGCTTCTTCTGATGGTTGGT	AATCGAACCCGCATCTTCTC	45.0	50.0	57.0	58.1	484 bp	1.0	72.0
psbZ-trnG-GCC_p2	TGCTTCTTCTGATGGTTGGT	GAATCGAACCCGCATCTTCT	45.0	50.0	57.0	58.1	485 bp	1.0	72.0
psbZ-trnG-GCC_p3	GCTTCTTCTGATGGTTGGTCA	AATCGAACCCGCATCTTCTC	47.6	50.0	58.2	58.1	483 bp	1.0	70.0
psbZ-trnG-GCC_p4	GCTTCTTCTGATGGTTGGTCA	GAATCGAACCCGCATCTTCT	47.6	50.0	58.2	58.1	484 bp	1.0	70.0
psbZ-trnG-GCC_p5	TGCTTCTTCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	47.6	50.0	58.2	58.1	484 bp	1.0	70.0