Hotspot

trnL-CAA-ycf15

IRb · 31170-31918 · 749 bp

Back to Goudaea

Pi 0

MarkerSeek score 61.60

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRb
length_bp: 749
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	GGCGAACAACCAGAGTACTC	50.0	55.0	58.0	58.3	913-935 bp	1.0	79.5
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	GGCGAACAACCAGAGTACTC	50.0	55.0	58.0	58.3	915-937 bp	1.0	79.5
trnL-CAA-ycf15_p3	AAATGGTAGACACGCGAGAC	GCGAACAACCAGAGTACTCA	50.0	50.0	58.0	57.6	912-934 bp	1.0	78.4
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	GCGAACAACCAGAGTACTCA	50.0	50.0	58.0	57.6	914-936 bp	1.0	78.4
trnL-CAA-ycf15_p5	TGGTGAAATGGTAGACACGC	GGCGAACAACCAGAGTACTC	50.0	55.0	58.2	58.3	918-940 bp	1.0	78.0