Hotspot

rpl32-trnL-UAG

SSC · 175-923 · 749 bp

Back to Goudaea

Pi 0.012

MarkerSeek score 55.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 749
variable_sites: 9
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	836-840 bp	1.0	76.8
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	837-841 bp	1.0	76.8
rpl32-trnL-UAG_p3	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	839-843 bp	1.0	76.3
rpl32-trnL-UAG_p4	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	840-844 bp	1.0	76.3
rpl32-trnL-UAG_p5	TTCCACCGGGCATTCAAAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.6	58.0	840-844 bp	1.0	76.3