Hotspot

rpl32-ndhF

SSC · 131033-132256 · 1224 bp

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Pi 0.0168

MarkerSeek score 55.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1224
variable_sites: 77
pi: 0.0168
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GGGGTTTTCTCCATTGGGTT	TGTTGCCGTCGGAAAAAGTA	50.0	45.0	58.0	58.0	1847-1885 bp	1.0	86.4
rpl32-ndhF_p2	GGGGTTTTCTCCATTGGGTT	GCCCACATACGACGAAGATT	50.0	50.0	58.0	58.1	1870-1908 bp	1.0	86.2
rpl32-ndhF_p3	TGGGCGATGGGGAAAATAAG	TGTTGCCGTCGGAAAAAGTA	50.0	45.0	57.9	58.0	1594-1616 bp	1.0	85.8
rpl32-ndhF_p4	TGGGCGATGGGGAAAATAAG	GCCCACATACGACGAAGATT	50.0	50.0	57.9	58.1	1617-1639 bp	1.0	85.6
rpl32-ndhF_p5	AAATGGGCGATGGGGAAAAT	TGTTGCCGTCGGAAAAAGTA	45.0	45.0	58.1	58.0	1598-1619 bp	0.823529	75.2