Hotspot

trnL-UAG-rpl32

SSC · 129740-130867 · 1128 bp

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Pi 0.016
MarkerSeek score 62.50
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1128
variable_sites
58
pi
0.016
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGAAAGGATATTGGGCGGC 50.0 50.0 58.0 58.3 1148-1268 bp 0.941176 83.0
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGAAAGGATATTGGGCGGC 50.0 50.0 58.0 58.3 1149-1269 bp 0.941176 82.9
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA AAGAAAGGATATTGGGCGGC 50.0 50.0 57.9 58.3 1158-1278 bp 0.941176 82.0
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC AAGAAAGGATATTGGGCGGC 55.0 50.0 57.8 58.3 1157-1277 bp 0.941176 81.4
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC AAGAAAGGATATTGGGCGGC 55.0 50.0 57.8 58.3 1156-1276 bp 0.941176 81.4