Hotspot

ndhC-trnV-UAC

LSC · 53718-54602 · 885 bp

Back to Gossypium

Pi 0.0127

MarkerSeek score 65.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 50
pi: 0.0127
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	57.8	57.9	1002-1088 bp	1.0	85.2
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	57.8	57.9	990-1076 bp	1.0	85.2
ndhC-trnV-UAC_p3	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	944-1030 bp	1.0	84.9
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	932-1018 bp	1.0	84.8
ndhC-trnV-UAC_p5	ATCGGGGCTAAAACTCCAGA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	58.4	57.9	992-1078 bp	1.0	83.4