Pi
0.0181
MarkerSeek score
66.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1416-1489 bp | 1.0 | 87.1 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1424-1497 bp | 1.0 | 86.5 |
| trnT-UGU-trnL-UAA_p3 | CAAATAGGGAAAGCCGGCTA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 1438-1511 bp | 1.0 | 86.3 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1388-1457 bp | 0.941176 | 85.4 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1396-1465 bp | 0.941176 | 84.9 |