Hotspot

rps4-trnT-UGU

LSC · 48105-48709 · 605 bp

Back to Gossypium

Pi 0.0244

MarkerSeek score 61.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 605
variable_sites: 42
pi: 0.0244
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	686-765 bp	1.0	87.9
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	690-769 bp	1.0	87.5
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	654-733 bp	1.0	87.0
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	658-737 bp	1.0	86.7
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	694-773 bp	1.0	84.9