Hotspot

trnE-UUC-trnT-GGU

LSC · 32080-32891 · 812 bp

Back to Gossypium

Pi 0.0125
MarkerSeek score 55.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
812
variable_sites
30
pi
0.0125
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCCTCCTTGAAAGAGAGGTG GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 911-945 bp 1.0 84.3
trnE-UUC-trnT-GGU_p2 GCCTCCTTGAAAGAGAGGTG CCATGGCGTTACTCTACCAC 55.0 55.0 57.9 58.1 890-924 bp 1.0 83.9
trnE-UUC-trnT-GGU_p3 GCCTCCTTGAAAGAGAGGTG TGGCGTTACTCTACCACTGA 55.0 50.0 57.9 58.1 887-921 bp 1.0 83.8
trnE-UUC-trnT-GGU_p4 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 952-986 bp 1.0 83.6
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 931-965 bp 1.0 83.3