Hotspot

petN-psbM

LSC · 29542-30667 · 1126 bp

Back to Gossypium

Pi 0.00654

MarkerSeek score 55.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 27
pi: 0.00654
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ATTGCTACAGCGCTGTTCAT	45.0	45.0	58.0	58.3	1020-1335 bp	1.0	83.6
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TATTGCTACAGCGCTGTTCA	45.0	45.0	58.0	57.6	1021-1336 bp	1.0	82.6
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	ATTGCTACAGCGCTGTTCAT	50.0	45.0	57.8	58.3	1026-1341 bp	1.0	82.1
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TTGCTACAGCGCTGTTCATT	45.0	45.0	58.0	58.5	1019-1334 bp	1.0	81.9
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TATTGCTACAGCGCTGTTCA	50.0	45.0	57.8	57.6	1027-1342 bp	1.0	81.2