Hotspot

atpH-atpI

LSC · 14072-15288 · 1217 bp

Back to Gossypium

Pi 0.0102

MarkerSeek score 53.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1217
variable_sites: 43
pi: 0.0102
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1300-1353 bp	1.0	83.3
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1334-1387 bp	1.0	83.2
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1270-1323 bp	1.0	83.1
atpH-atpI_p4	AATAGAAGCAAGCCCGACAG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1304-1357 bp	1.0	83.0
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1356-1409 bp	1.0	82.5