Hotspot

atpF-atpH

LSC · 13206-13825 · 620 bp

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Pi 0.0112

MarkerSeek score 56.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 21
pi: 0.0112
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	50.0	50.0	58.0	58.5	678-706 bp	1.0	82.7
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTC	50.0	50.0	58.0	58.5	677-705 bp	1.0	82.5
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	GGTTGTAGCATTGGCACTCT	50.0	50.0	58.0	58.2	676-704 bp	0.941176	82.4
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	50.0	50.0	58.0	58.5	683-711 bp	1.0	82.2
atpF-atpH_p5	AGTGACCCAAGGAAACGAAA	GGTTGTAGCATTGGCACTCT	45.0	50.0	57.3	58.2	675-703 bp	0.941176	76.9