Hotspot

rps16-trnQ-UUG

LSC · 6307-6892 · 586 bp

Back to Gossypium

Pi 0.0216

MarkerSeek score 64.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 48
pi: 0.0216
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	692-707 bp	1.0	89.0
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	653-668 bp	1.0	88.2
rps16-trnQ-UUG_p3	AGTGGTGGGTTTTGACATCC	CTATGCGGAGGTTCGAATCC	50.0	55.0	58.0	58.2	699-714 bp	1.0	87.8
rps16-trnQ-UUG_p4	AACGGAAAGTGGTGGGTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	699-714 bp	1.0	87.8
rps16-trnQ-UUG_p5	CATAACGGAAAGTGGTGGGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	702-717 bp	1.0	87.4