Hotspot

trnK-UUU-rps16

LSC · 4340-5171 · 832 bp

Back to Gossypium

Pi 0.017

MarkerSeek score 56.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 48
pi: 0.017
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	964-1058 bp	1.0	87.9
trnK-UUU-rps16_p2	CCATTGCTACGACATGCTCT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.1	58.0	998-1092 bp	1.0	87.6
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	1103-1197 bp	1.0	87.3
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	1104-1198 bp	1.0	87.3
trnK-UUU-rps16_p5	AGACAGGCGGATTCATCAAC	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.0	58.0	1485-1577 bp	0.941176	84.9