Hotspot

rpl32-trnL-UAG

SSC · 115978-116898 · 921 bp

Back to Gordonia

Pi 0.0396

MarkerSeek score 72.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 921
variable_sites: 55
pi: 0.0396
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGAAATCTCTTTCAAGCGG	GACGGAATGGAAATCGGGAA	50.0	50.0	56.8	58.0	1126 bp	0.25	40.0
rpl32-trnL-UAG_p2	GGGAAATCTCTTTCAAGCGG	ATGACGGAATGGAAATCGGG	50.0	50.0	56.8	58.0	1128 bp	0.25	40.0
rpl32-trnL-UAG_p3	AGGGAAATCTCTTTCAAGCGG	GACGGAATGGAAATCGGGAA	47.6	50.0	58.3	58.0	1127 bp	0.25	39.5
rpl32-trnL-UAG_p4	AGGGAAATCTCTTTCAAGCGG	ATGACGGAATGGAAATCGGG	47.6	50.0	58.3	58.0	1129 bp	0.25	39.5
rpl32-trnL-UAG_p5	GGGAAATCTCTTTCAAGCGGA	GACGGAATGGAAATCGGGAA	47.6	50.0	58.3	58.0	1126 bp	0.25	39.4