Hotspot

rps8-rpl14

LSC · 83133-83321 · 189 bp

Back to Gordonia

Pi 0.0476

MarkerSeek score 75.20

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 14
pi: 0.0476
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	GCCCGAGAATTGAGACAGTT	50.0	50.0	57.7	57.9	273-280 bp	1.0	82.3
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	TCATTGGCTCCCGAGGTATT	50.0	50.0	57.7	58.5	234-241 bp	1.0	80.0
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	TTCATTGGCTCCCGAGGTAT	50.0	50.0	57.7	58.5	235-242 bp	1.0	80.0
rps8-rpl14_p4	CAGCAATAGTGTCCCTACCC	GCCCGAGAATTGAGACAGTT	55.0	50.0	57.4	57.9	274-281 bp	1.0	79.4
rps8-rpl14_p5	AGCAATAGTGTCCCTACCCA	TAGTTTCATTGGCTCCCGAG	50.0	50.0	57.7	57.3	239-246 bp	1.0	78.3