Hotspot

psbT-psbN

LSC · 76841-76900 · 60 bp

Back to Gordonia

Pi 0.0472

MarkerSeek score 72.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.0472
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	TCGAGGAACATGGGGACTAA	50.0	50.0	57.6	57.8	120 bp	1.0	77.9
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	TTCGAGGAACATGGGGACTA	50.0	50.0	57.6	57.8	121 bp	1.0	77.8
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	CATTCGAGGAACATGGGGAC	50.0	55.0	57.6	58.3	123 bp	1.0	77.1
psbT-psbN_p4	GAGAACCGCCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	50.0	50.0	57.5	57.8	118 bp	1.0	77.0
psbT-psbN_p5	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	175 bp	1.0	76.7