Hotspot

trnT-GGU-psbD

LSC · 33237-34755 · 1519 bp

Back to Gordonia

Pi 0.0334
MarkerSeek score 72.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1519
variable_sites
79
pi
0.0334
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1565-1590 bp 1.0 46.9
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1588-1613 bp 1.0 46.8
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1587-1612 bp 1.0 46.8
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1591-1616 bp 1.0 46.7
trnT-GGU-psbD_p5 GGCCCTTTTAACTCAGTGGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.7 53.0 1603-1628 bp 1.0 46.7