Hotspot

rpoB-trnC-GCA

LSC · 27817-29058 · 1242 bp

Back to Gordonia

Pi 0.0355

MarkerSeek score 73.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1242
variable_sites: 72
pi: 0.0355
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCATTTCCATCCCGTAGCA	AAAAGGATTTGCAGTCCCCC	47.6	50.0	58.1	58.4	1279-1308 bp	1.0	75.6
rpoB-trnC-GCA_p2	TTCATTTCCATCCCGTAGCA	AAAAGGATTTGCAGTCCCCC	45.0	50.0	56.9	58.4	1278-1307 bp	1.0	75.5
rpoB-trnC-GCA_p3	TTCCTTCATTTCCATCCCGT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	56.8	58.4	1282-1311 bp	1.0	74.7
rpoB-trnC-GCA_p4	TCATTTCCATCCCGTAGCAT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	56.7	58.4	1277-1306 bp	1.0	73.7
rpoB-trnC-GCA_p5	TTCATTTCCATCCCGTAGCAT	AAAAGGATTTGCAGTCCCCC	42.9	50.0	57.4	58.4	1278-1307 bp	1.0	71.7