Hotspot

atpF-atpH

LSC · 13859-14227 · 369 bp

Back to Gordonia

Pi 0.0407

MarkerSeek score 77.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 24
pi: 0.0407
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GGACTGGTTGTTGCATTAGC	50.0	50.0	57.9	57.4	486-528 bp	1.0	78.2
atpF-atpH_p2	CCAGTAGCCCAAAGAAACGA	GGACTGGTTGTTGCATTAGC	50.0	50.0	57.8	57.4	459-501 bp	1.0	78.0
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	551-593 bp	1.0	77.3
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	552-594 bp	1.0	77.2
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	553-595 bp	1.0	77.2