Hotspot

trnG-trnR-UCU

LSC · 10563-10819 · 257 bp

Back to Gordonia

Pi 0.0473

MarkerSeek score 73.40

Primer available yes

Label trnG-trnR-UCU

Feature Metadata

label_name: trnG-trnR-UCU
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 18
pi: 0.0473
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR-UCU_p1	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	314-335 bp	1.0	48.4
trnG-trnR-UCU_p2	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	313-334 bp	1.0	48.4
trnG-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	325-346 bp	1.0	48.3
trnG-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	55.0	38.1	58.1	52.4	328-349 bp	1.0	48.3
trnG-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	329-350 bp	1.0	48.2