Hotspot

rps16-trnQ-UUG

LSC · 6179-7792 · 1614 bp

Back to Gordonia

Pi 0.0366

MarkerSeek score 71.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1614
variable_sites: 92
pi: 0.0366
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATGAAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1762-1857 bp	1.0	87.5
rps16-trnQ-UUG_p2	AAAGGGCGTTTCCTTGTTCT	GAAATGAAAATGGGGCGTGG	45.0	50.0	57.9	58.0	2230-2315 bp	1.0	87.3
rps16-trnQ-UUG_p3	TTGAGCGGTCTTTAAACCCC	GAAATGAAAATGGGGCGTGG	50.0	50.0	58.1	58.0	2320-2405 bp	1.0	87.0
rps16-trnQ-UUG_p4	AAAGGGCGTTTCCTTGTTCT	CGAAAACTTACGGCAGCTTG	45.0	50.0	57.9	58.0	2735-2820 bp	1.0	85.9
rps16-trnQ-UUG_p5	CTACTCGAGCTTCATCGTGG	GAAATGAAAATGGGGCGTGG	55.0	50.0	57.9	58.0	1895-1912 bp	0.5	59.5