Hotspot

ycf1

SSC · 123573-128936 · 5364 bp

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Pi 0.0357

MarkerSeek score 72.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5364
variable_sites: 666
pi: 0.0357
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATATGCTTGCGACAGGGAAG	TCGGATTTTCGTCGCAATCT	50.0	45.0	58.0	58.0	1386-1494 bp	1.0	91.5
ycf1_p2	TACATATGCTTGCGACAGGG	TCGGATTTTCGTCGCAATCT	50.0	45.0	57.8	58.0	1389-1497 bp	0.818182	82.6
ycf1_p3	GCTTGCGACAGGGAAGATAA	TCGGATTTTCGTCGCAATCT	50.0	45.0	58.0	58.0	1382-1490 bp	0.727273	80.4
ycf1_p4	GCTTGCGACAGGGAAGATAA	CTTCCCTTTGGTTCTCCTCG	50.0	55.0	58.0	57.9	728 bp	0.090909	42.6
ycf1_p5	ATATGCTTGCGACAGGGAAG	CTTCCCTTTGGTTCTCCTCG	50.0	55.0	58.0	57.9	732 bp	0.090909	42.5