Hotspot

accD

LSC · 56317-58032 · 1716 bp

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Pi 0.0471

MarkerSeek score 78.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1716
variable_sites: 181
pi: 0.0471
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGATCAAGCCAAGGGAAGGA	ATCCGGGGCAAAAAGTCATT	50.0	45.0	58.3	58.1	2032-2466 bp	0.636364	74.2
accD_p2	AGATCAAGCCAAGGGAAGGA	TCAGCAAGAGAGGTGGAAGA	50.0	50.0	58.3	58.0	2113-2505 bp	0.636364	73.4
accD_p3	GCCATAGATCAAGCCAAGGG	TCAGCAAGAGAGGTGGAAGA	55.0	50.0	58.4	58.0	2118-2510 bp	0.636364	72.9
accD_p4	TCCTTGGGAATGGTGGATCA	ATCCGGGGCAAAAAGTCATT	50.0	45.0	58.3	58.1	2076-2404 bp	0.272727	55.6
accD_p5	TCCTTGGGAATGGTGGATCA	TCAGCAAGAGAGGTGGAAGA	50.0	50.0	58.3	58.0	2159-2443 bp	0.272727	54.7