Hotspot

rpl32-trnL-UAG

SSC · 113147-113891 · 745 bp

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Pi 0.0505

MarkerSeek score 84.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 745
variable_sites: 123
pi: 0.0505
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTGACATAGAAGTACGTTTTT	TCCTAAGAGCAGCGTGTCTA	27.3	50.0	52.0	57.9	721-1043 bp	1.0	52.0
rpl32-trnL-UAG_p2	TTTTGACATAGAAGTACGTTTT	TCCTAAGAGCAGCGTGTCTA	27.3	50.0	52.0	57.9	722-1044 bp	1.0	52.0
rpl32-trnL-UAG_p3	TTTTTGACATAGAAGTACGTTT	TCCTAAGAGCAGCGTGTCTA	27.3	50.0	52.0	57.9	723-1045 bp	1.0	52.0
rpl32-trnL-UAG_p4	TTTGACATAGAAGTACGTTTTT	AGCAGCGTGTCTACCAATTT	27.3	45.0	52.0	57.8	714-1036 bp	1.0	52.0
rpl32-trnL-UAG_p5	TTTTGACATAGAAGTACGTTTT	AGCAGCGTGTCTACCAATTT	27.3	45.0	52.0	57.8	715-1037 bp	1.0	52.0