Hotspot

rpl16-rps3

LSC · 82329-82498 · 170 bp

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Pi 0.0453

MarkerSeek score 80.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 25
pi: 0.0453
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.1	58.0	302-319 bp	1.0	83.9
rpl16-rps3_p2	ACGAGTCACACACTAAGCAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	56.9	58.0	301-318 bp	1.0	82.1
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	CCCTTACAAACAATTCGCGC	45.0	50.0	57.1	58.4	294-311 bp	1.0	81.1
rpl16-rps3_p4	AACGAGTCACACACTAAGCAT	GCAGGGTTCCCTTACAAACA	42.9	50.0	57.6	58.0	302-319 bp	1.0	79.9
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	CCTTACAAACAATTCGCGCT	45.0	45.0	57.1	57.4	293-310 bp	1.0	79.3