Hotspot

ndhC-trnV-UAC

LSC · 49621-50250 · 630 bp

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Pi 0.0538

MarkerSeek score 88.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 124
pi: 0.0538
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCAAGATAGGAATAACGCT	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.2	57.8	735-811 bp	1.0	75.7
ndhC-trnV-UAC_p2	TGCCAAGATAGGAATAACGCTT	AGAAGGTCTACGGTTCGAGT	40.9	50.0	57.8	57.8	735-811 bp	1.0	73.2
ndhC-trnV-UAC_p3	TGCCAAGATAGGAATAACGCT	GAAGGTCTACGGTTCGAGTC	42.9	55.0	57.2	57.5	734-810 bp	1.0	73.0
ndhC-trnV-UAC_p4	TGCCAAGATAGGAATAACGCT	GTTCGAGTCCGTATAGCCCT	42.9	55.0	57.2	58.7	723-799 bp	1.0	71.8
ndhC-trnV-UAC_p5	ATGCCAAGATAGGAATAACGCT	AGAAGGTCTACGGTTCGAGT	40.9	50.0	57.6	57.8	736-812 bp	1.0	71.6