Hotspot

trnF-GAA-ndhJ

LSC · 47400-47915 · 516 bp

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Pi 0.0433

MarkerSeek score 85.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 79
pi: 0.0433
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	620-731 bp	1.0	90.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	610-721 bp	1.0	90.0
trnF-GAA-ndhJ_p3	ATTGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	663-774 bp	1.0	89.3
trnF-GAA-ndhJ_p4	ATTGGTCGGGATAGCTCAGT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.2	58.2	653-764 bp	1.0	88.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TAGGCTGGCCCTTACGTAAA	50.0	50.0	58.1	58.4	609-720 bp	1.0	88.2