Hotspot

trnL-UAA-trnF-GAA

LSC · 46909-47326 · 418 bp

Back to Goodyera

Pi 0.0605

MarkerSeek score 84.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 67
pi: 0.0605
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	452-520 bp	1.0	87.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	414-482 bp	1.0	84.6
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	449-517 bp	1.0	84.5
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	441-509 bp	1.0	83.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	424-492 bp	1.0	83.4