Hotspot

psaA-ycf3

LSC · 40846-41472 · 627 bp

Back to Goodyera

Pi 0.0311

MarkerSeek score 82.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 70
pi: 0.0311
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	ATAGCGCTTACTCCAGGGAA	50.0	50.0	58.5	58.2	706-825 bp	1.0	86.2
psaA-ycf3_p2	TGTTCTTGAGAAATGGCCGG	TAGCGCTTACTCCAGGGAAT	50.0	50.0	58.5	58.2	705-824 bp	1.0	86.2
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	680-799 bp	0.909091	84.9
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	679-798 bp	0.909091	84.9
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	683-802 bp	0.909091	83.7