Hotspot

matK-trnK-UUU

LSC · 3221-4295 · 1075 bp

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Pi 0.0461

MarkerSeek score 82.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 168
pi: 0.0461
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CTAGATGGATGGGATGGGGT	ATCCGGGTTGCTAACTCAAC	55.0	50.0	57.9	57.9	1476-1622 bp	1.0	90.7
matK-trnK-UUU_p2	CTAGATGGATGGGATGGGGT	AACGGTAGAGTACTCGGCTT	55.0	50.0	57.9	58.2	1459-1605 bp	1.0	90.2
matK-trnK-UUU_p3	CTAGATGGATGGGATGGGGT	ACGGTAGAGTACTCGGCTTT	55.0	50.0	57.9	58.2	1458-1604 bp	1.0	90.2
matK-trnK-UUU_p4	TCTAGATGGATGGGATGGGG	ATCCGGGTTGCTAACTCAAC	55.0	50.0	57.7	57.9	1477-1623 bp	1.0	88.3
matK-trnK-UUU_p5	CTAGATGGATGGGATGGGGT	GGGTTGCTAACTCAACGGTA	55.0	50.0	57.9	57.5	1472-1618 bp	1.0	87.9