Hotspot

rps14

LSC · 39221-39523 · 303 bp

Back to Gonystylus

Pi 0.0033

MarkerSeek score 42.60

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 1
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	AGAAATAGAAAAATGAAGTGACA	GTGCCGTATCCGCGATAATA	26.1	50.0	52.3	57.7	449 bp	1.0	40.2
rps14_p2	AGAAAGAAATAGAAAAATGAAGTGACA	GTGCCGTATCCGCGATAATA	25.9	50.0	56.2	57.7	453 bp	1.0	40.2
rps14_p3	AAGAAATAGAAAAATGAAGTGACA	GTGCCGTATCCGCGATAATA	25.0	50.0	53.1	57.7	450 bp	1.0	40.2
rps14_p4	GAAAGAAATAGAAAAATGAAGTGACA	GTGCCGTATCCGCGATAATA	26.9	50.0	55.0	57.7	452 bp	1.0	40.2
rps14_p5	AGAAATAGAAAAATGAAGTGACAT	GTGCCGTATCCGCGATAATA	25.0	50.0	52.9	57.7	449 bp	1.0	40.2