Hotspot

rpl20

LSC · 72991-73389 · 399 bp

Back to Gonystylus

Pi 0.013

MarkerSeek score 58.40

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 5
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	GAGTTCATTCTCGAGGGAAC	TCATCAAAGTTTCTTTTCGTCA	50.0	31.8	55.6	54.5	453-468 bp	1.0	43.5
rpl20_p2	ACCTTCTCCGAGTTCATTCTC	TCATCAAAGTTTCTTTTCGTCA	47.6	31.8	57.1	54.5	462-477 bp	1.0	43.4
rpl20_p3	ACCTTCTCCGAGTTCATTCTC	TTCATCAAAGTTTCTTTTCGTCA	47.6	30.4	57.1	55.3	463-478 bp	1.0	43.4
rpl20_p4	ACCTTCTCCGAGTTCATTCT	TCATCAAAGTTTCTTTTCGTCA	45.0	31.8	55.9	54.5	462-477 bp	1.0	43.4
rpl20_p5	ACCTTCTCCGAGTTCATTCT	TTCATCAAAGTTTCTTTTCGTCA	45.0	30.4	55.9	55.3	463-478 bp	1.0	43.4