Hotspot

rps12-rps7

IRa · 162162-162793 · 632 bp

Back to Gonystylus

Pi 0

MarkerSeek score 63.60

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 632
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	CCCTAGATGCTGTCGGAGTA	TTTTTCTGCAGTACCTCGGC	55.0	50.0	58.0	58.5	683-715 bp	1.0	79.7
rps12-rps7_p2	AGGATTTACCCGGTGTGAGA	TTTTTCTGCAGTACCTCGGC	50.0	50.0	58.1	58.5	722-754 bp	1.0	79.2
rps12-rps7_p3	GAGGGGGAAGGGTTAAGGAT	TTTTTCTGCAGTACCTCGGC	55.0	50.0	58.1	58.5	737-769 bp	1.0	78.8
rps12-rps7_p4	GCTGTCGGAGTAAAGGATCG	TTTTTCTGCAGTACCTCGGC	55.0	50.0	58.2	58.5	675-707 bp	1.0	78.6
rps12-rps7_p5	ACCCGGTGTGAGATATCACA	TTTTTCTGCAGTACCTCGGC	50.0	50.0	58.1	58.5	715-747 bp	1.0	78.5