Hotspot

ndhF-rpl32

SSC · 133271-134135 · 865 bp

Back to Gonystylus

Pi 0.00698

MarkerSeek score 63.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 865
variable_sites: 6
pi: 0.00698
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGAAAAAGCAGAAGTCCC	CTTTCAACGCTGCCCAATAC	50.0	50.0	58.1	57.7	1047-1059 bp	1.0	77.9
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	CTTTCAACGCTGCCCAATAC	45.0	50.0	57.8	57.7	1057-1069 bp	1.0	77.2
ndhF-rpl32_p3	TCGGAAAAAGCAGAAGTCCC	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.1	58.4	1053-1065 bp	1.0	76.6
ndhF-rpl32_p4	AAAAGCAGAAGTCCCACTCC	CTTTCAACGCTGCCCAATAC	50.0	50.0	57.7	57.7	1042-1054 bp	1.0	76.6
ndhF-rpl32_p5	AAGCAGAAGTCCCACTCCTA	CTTTCAACGCTGCCCAATAC	50.0	50.0	57.7	57.7	1040-1052 bp	1.0	76.2