Hotspot

rpl33-rps18

LSC · 72063-72377 · 315 bp

Back to Gonystylus

Pi 0.0127

MarkerSeek score 60.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 315
variable_sites: 4
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GCCCAGTCGATTGGAATTGA	CAAACGCCTACGCAAAGATC	50.0	50.0	58.2	57.8	448-464 bp	1.0	77.4
rpl33-rps18_p2	GCCCAGTCGATTGGAATTGA	AAGAAAGAGCCGCTTGGATT	50.0	45.0	58.2	57.8	418-434 bp	1.0	77.1
rpl33-rps18_p3	GCCCAGTCGATTGGAATTGA	AGAAAGAGCCGCTTGGATTT	50.0	45.0	58.2	57.8	417-433 bp	1.0	77.1
rpl33-rps18_p4	GCCCAGTCGATTGGAATTGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.2	57.7	477-493 bp	1.0	76.6
rpl33-rps18_p5	GCCCAGTCGATTGGAATTGA	TACGCAAAGATCGCTTGGAT	50.0	45.0	58.2	57.7	440-456 bp	1.0	76.2