Hotspot

accD-psaI

LSC · 62314-63136 · 823 bp

Back to Gonystylus

Pi 0.00746

MarkerSeek score 59.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 6
pi: 0.00746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGATCCAATCATACCACGCA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	984-993 bp	1.0	73.8
accD-psaI_p2	TGATCCAATCATACCACGCA	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	990-999 bp	1.0	73.2
accD-psaI_p3	TGATCCAATCATACCACGCAA	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.7	58.0	984-993 bp	1.0	71.6
accD-psaI_p4	TTGATCCAATCATACCACGCA	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.7	58.0	985-994 bp	1.0	71.6
accD-psaI_p5	TGATCCAATCATACCACGCAA	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.7	57.9	990-999 bp	1.0	71.0