Hotspot

trnF-GAA-ndhJ

LSC · 51670-52393 · 724 bp

Back to Gonystylus

Pi 0.00552

MarkerSeek score 61.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 4
pi: 0.00552
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CATCCACGACTGAAGCGTAT	55.0	50.0	58.0	57.8	899-911 bp	1.0	78.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAGCGTAT	50.0	50.0	58.1	57.8	863-875 bp	1.0	78.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	CCACGACTGAAGCGTATCTT	55.0	50.0	58.0	57.7	896-908 bp	1.0	78.1
trnF-GAA-ndhJ_p4	TGGTAATGGTCGGGATAGCT	CATCCACGACTGAAGCGTAT	50.0	50.0	57.9	57.8	910-922 bp	1.0	77.9
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAGCGTATCTT	50.0	50.0	58.1	57.7	860-872 bp	1.0	77.7