Hotspot

rpoB-trnC-GCA

LSC · 27984-29098 · 1115 bp

Back to Gonystylus

Pi 0.00538

MarkerSeek score 61.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1115
variable_sites: 6
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCATTTTCATCCCCGAGCAT	CTGGGGTAAAGGGATTTGCA	50.0	50.0	58.3	57.8	1186-1187 bp	1.0	76.1
rpoB-trnC-GCA_p2	CCATTTTCATCCCCGAGCAT	TGGGGTAAAGGGATTTGCAG	50.0	50.0	58.3	57.8	1185-1186 bp	1.0	76.1
rpoB-trnC-GCA_p3	CCATTTTCATCCCCGAGCAT	ACTGGGGTAAAGGGATTTGC	50.0	50.0	58.3	57.8	1187-1188 bp	1.0	76.1
rpoB-trnC-GCA_p4	CCATTTTCATCCCCGAGCAT	GGGGTAAAGGGATTTGCAGT	50.0	50.0	58.3	57.8	1184-1185 bp	1.0	76.1
rpoB-trnC-GCA_p5	ATTCCCCCATTTTCATCCCC	TGGGGTAAAGGGATTTGCAG	50.0	50.0	57.5	57.8	1191-1192 bp	1.0	74.6