Hotspot

chlL-ycf2

Genome · 127481-128526 · 1046 bp

Back to Goniophlebium

Pi 0.164

MarkerSeek score 82.50

Primer available yes

Label chlL-ycf2

Feature Metadata

label_name: chlL-ycf2
feature_type: igs
region: Genome
length_bp: 1046
variable_sites: 168
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-ycf2_p1	TCTCCCACGACATATCCTCC	TCGCTTTGATTCCTACGTGG	55.0	50.0	58.0	58.0	1568-1577 bp	1.0	88.9
chlL-ycf2_p2	TCTGCGTAATTAAGTGGGGC	TCGCTTTGATTCCTACGTGG	50.0	50.0	58.0	58.0	1682-1691 bp	1.0	88.6
chlL-ycf2_p3	TCTCCCACGACATATCCTCC	ACATTTAATCCCGCTCGCTT	55.0	45.0	58.0	58.0	1582-1591 bp	1.0	88.6
chlL-ycf2_p4	TCTCCCACGACATATCCTCC	TCCGCAGACATTTAATCCCG	55.0	50.0	58.0	58.1	1589-1598 bp	1.0	88.5
chlL-ycf2_p5	TCTGCGTAATTAAGTGGGGC	ACATTTAATCCCGCTCGCTT	50.0	45.0	58.0	58.0	1696-1705 bp	1.0	88.3