Hotspot

rpl32-trnP-GGG

Genome · 109058-109676 · 619 bp

Back to Goniophlebium

Pi 0.173
MarkerSeek score 79.10
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
Genome
length_bp
619
variable_sites
107
pi
0.173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 CAATTCACTTCGGCGGTAGA GAGGTCACAGGTTCGAATCC 50.0 55.0 58.0 58.0 1057 bp 0.5 59.9
rpl32-trnP-GGG_p2 CAATTCACTTCGGCGGTAGA GGAGGTCACAGGTTCGAATC 50.0 55.0 58.0 58.0 1058 bp 0.5 59.9
rpl32-trnP-GGG_p3 TCAATTCACTTCGGCGGTAG GAGGTCACAGGTTCGAATCC 50.0 55.0 58.0 58.0 1058 bp 0.5 59.9
rpl32-trnP-GGG_p4 TCAATTCACTTCGGCGGTAG GGAGGTCACAGGTTCGAATC 50.0 55.0 58.0 58.0 1059 bp 0.5 59.9
rpl32-trnP-GGG_p5 GAGATTCTACGACGCTCGAC GAGGTCACAGGTTCGAATCC 55.0 55.0 58.0 58.0 1320 bp 0.5 59.8