Hotspot

psbD-trnT-GGU

Genome · 32982-34335 · 1354 bp

Back to Goniophlebium

Pi 0.107

MarkerSeek score 82.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1354
variable_sites: 143
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	45.0	55.0	58.1	58.5	1439-1492 bp	1.0	84.4
psbD-trnT-GGU_p2	TTTCCGATGGCAATGGTCAT	AACTCAGGGGTAGAGTGACG	45.0	55.0	57.9	58.5	1438-1491 bp	1.0	83.9
psbD-trnT-GGU_p3	TTTTCCGATGGCAATGGTCA	GCCATGGTAAGGCGTAAGTC	45.0	55.0	58.1	58.7	1420-1473 bp	1.0	82.5
psbD-trnT-GGU_p4	TTTCCGATGGCAATGGTCAT	GCCATGGTAAGGCGTAAGTC	45.0	55.0	57.9	58.7	1419-1472 bp	1.0	82.0
psbD-trnT-GGU_p5	TTTTCCGATGGCAATGGTCA	GGTTCAAATCCGACAAGGGG	45.0	55.0	58.1	58.8	1397-1450 bp	1.0	81.6