Hotspot

atpH-atpI

Genome · 12349-13084 · 736 bp

Back to Goniophlebium

Pi 0.112

MarkerSeek score 82.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 736
variable_sites: 80
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GTAGGAAGTGACAAGCCTCG	CATTCAGTTACCCCACGGAG	55.0	55.0	58.0	58.0	1644-1665 bp	1.0	88.2
atpH-atpI_p2	GTAGGAAGTGACAAGCCTCG	AGGTGCCAGAAATCTGCAAA	55.0	45.0	58.0	58.0	1836-1857 bp	1.0	87.7
atpH-atpI_p3	TTCTGGCTGTCTCGCAATAC	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1430-1448 bp	1.0	87.5
atpH-atpI_p4	GAGGGAGTAGCGATCTCACT	AGGTGCCAGAAATCTGCAAA	55.0	45.0	58.0	58.0	1924-1945 bp	1.0	87.2
atpH-atpI_p5	GGAGGGGTAAAAGTGCAACA	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1605 bp	0.5	59.9